Amen · Journal of Alzheimer's disease : JAD 2016 · Case-control diagnostic study · n=285

Perfusion Neuroimaging Abnormalities Alone Distinguish National Football League Players from a Healthy Population.

Cited 53 times in the scientific literature.

Level 4 - case-series / case-control

Case-control diagnostic imaging study comparing NFL players to separately recruited healthy controls.

PubMed 27128374 · doi:10.3233/JAD-160207 · record verified 2026-08-29

What was done

Researchers evaluated 161 current and retired National Football League (NFL) players and 124 separately recruited healthy controls. Participants underwent medical evaluations, neuropsychological testing, and single-photon emission computed tomography (SPECT) to quantify regional cerebral perfusion using a standard atlas. Differences in regional perfusion were tested via one-way ANOVA, and predictive classification models (discriminant analysis and automatic linear modeling) were used to test how accurately perfusion patterns alone differentiated players from controls.

What was found

NFL players exhibited significantly lower average cerebral perfusion across 36 brain regions (p < 0.01) compared to healthy controls. Discriminant analysis separated NFL players from controls with 90% sensitivity, 86% specificity, and 94% accuracy (reported 95% CI 95-99). Automatic linear modeling yielded similar performance, and adding age or clinical comorbidities did not improve diagnostic classification.

Why it matters

This study shows that widespread regional cerebral hypoperfusion detected by SPECT correlates with a history of professional football play and repetitive head trauma, providing a potential imaging biomarker profile to distinguish exposed players from healthy non-athletes.

Limits

The use of separately recruited healthy controls rather than non-contact athletic controls risks spectrum bias and confounding from athletic or lifestyle differences. The abstract does not correlate hypoperfusion with cumulative concussion counts, career duration, or specific clinical symptomatology, nor does it establish whether these perfusion abnormalities precede or predict neurodegenerative disease.

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