DavidPerlmutterMD · 2021-02-25 · David Perlmutter (host), Daniel Amen

Recognize Influences on the Brain - with Dr. Daniel Amen | The Empowering Neurologist EP. 114

28 research-tied claims examined: 1 contradicted 1 contradicted online 4 context 18 supported 2 corroborated online 2 unverified

1

Contradicted by research

0:56:00Daniel Amencontradictedmoderate

Low blood flow is the number one brain imaging predictor of Alzheimer's disease.

"Blood flow—low blood flow is the number one brain imaging predictor of Alzheimer's disease." (said at 0:56:00)

Low cerebral blood flow (hypoperfusion, typically assessed via SPECT or arterial spin labeling MRI) is not the leading or "number one" neuroimaging predictor of Alzheimer's disease. In clinical diagnostic frameworks and comparative imaging studies, pathological hallmark imaging (amyloid-beta PET and tau PET), fluorodeoxyglucose (FDG) PET, and structural MRI (such as medial temporal lobe and hippocampal volumetry) are the primary, most accurate imaging biomarkers for Alzheimer's disease. While characteristic patterns of hypoperfusion (particularly in the posterior cingulate and temporoparietal cortices) are observed in Alzheimer's disease and can assist in differential diagnosis or prognostication of mild cognitive impairment, systematic reviews and comparative studies demonstrate that FDG-PET and specific amyloid/tau imaging biomarkers consistently demonstrate higher diagnostic accuracy and predictive power than perfusion imaging.

1

Contradicted by web sources

0:47:48David Perlmutter (host)contradicted (web)

About 70% of evening news television advertisements are for direct-to-consumer monoclonal antibody therapies for autoimmune or hyperimmune conditions.

"If you don't think that's the case, watch the evening news and see what about 70% of the advertisements are for: they're for monoclonal antibody-based direct-to-consumer drug platforms to get you to ask your doctor about this monoclonal antibody for this particular hyper/autoimmune immune condition." (said at 0:47:48)

No published record matching the claim that approximately 70% of evening news television advertisements are for direct-to-consumer monoclonal antibody therapies for autoimmune or hyperimmune conditions was located; this does not prove the claim false. While pharmaceutical direct-to-consumer advertising is common during broadcast television news programming, no empirical content analysis or media monitoring study in the published literature documents this specific proportion for monoclonal antibody therapies targeting autoimmune diseases.

contradicted by web sources

Industry advertising tracking and news media analyses report that all pharmaceutical advertisements combined account for roughly 24% to 30% of ad time during network evening newscasts. Monoclonal antibody therapies for autoimmune conditions represent only a fraction of those pharmaceutical commercials, alongside treatments for diabetes, cardiovascular disease, weight management, and other conditions.

wisconsinwatch.orgsenate.govstudyfinds.com

4

Needs context

0:47:15David Perlmutter (host)needs contextmoderate

The hygiene hypothesis, described in 1988, posits that reduced microbial exposure leads to immune imbalances that drive hyperimmune and autoimmune conditions like childhood food allergies.

"So you know, there is something called the hygiene hypothesis that was described in 1988 whereby our obsession with hygiene is really leading to imbalance of our immune responsiveness, such that we have hyperimmune, autoimmune conditions rampant today, like food allergies in children and autoimmune conditions that are really quite prevalent in adults." (said at 0:47:15)

The host accurately describes the core premise of the hygiene hypothesis—that reduced early-life microbial exposure due to modern hygienic conditions leads to immune dysregulation, increasing susceptibility to allergic and autoimmune conditions. However, the landmark paper by David Strachan that formulated this hypothesis was published in 1989 (not 1988) and initially focused on hay fever and allergic sensitization, though subsequent research expanded the model to encompass broader autoimmune disorders.

  • context: The hygiene hypothesis for allergy - conception and evolution. (Frontiers in allergy 2022) · cited 59x in the literature
    "In 1989, a short paper entitled "Hay fever, hygiene and household size" observed that British children from larger families were less likely to develop hay fever and suggested that this could be because early exposure to infection prevents allergy. This sibship size association for hay fever, since replicated many times in Britain and other affluent countries and confirmed by objective measures of atopy, prompted what has come to be known as the "hygiene hypothesis for allergy", although that term was not specifically used in the 1989 paper." (abstract, passage verified)
    pubmedfull study (doi)
0:56:10Daniel Amenneeds contextmoderate

Forty percent of 40-year-old men begin to complain of erectile dysfunction.

"And get this: 40% of men at 40 years old start to complain of erectile dysfunction." (said at 0:56:10)

Data from the landmark Massachusetts Male Aging Study (MMAS) established that approximately 40% of men at age 40 experience some degree of erectile dysfunction (with an overall prevalence of 52% across men aged 40 to 70). However, this figure includes mild or minimal erectile difficulties; severe or complete erectile dysfunction affects only about 5% of men at age 40, increasing with age.

0:58:25David Perlmutter (host)needs contextmoderate

By the time fasting blood sugar becomes elevated, a person has experienced pancreatic exhaustion of insulin production for at least a decade.

"When your fasting blood sugar is elevated, you've now exhausted your pancreas' ability to manufacture enough insulin to keep your blood sugar normal. That's been going on for a decade at least." (said at 0:58:25)

The claim correctly identifies that metabolic dysregulation precedes elevated fasting glucose by a decade or more, but mischaracterizes the pancreatic response during that timeframe. In the early stages of insulin resistance, the pancreas does not suffer from insulin exhaustion; rather, it compensates by hyper-secreting insulin (hyperinsulinemia) to maintain normal blood glucose. Prospective longitudinal cohort data show that markers of insulin secretion (such as HOMA2-B) actually increase up until approximately 7 years prior to a diabetes diagnosis, after which beta-cell secretion begins to decline. Thus, while metabolic dysfunction is long-standing, true failure or exhaustion of insulin production does not persist for a decade prior to the elevation of fasting glucose.

0:59:30Daniel Amenneeds contextlow

A study by Daniel Amen in the Journal of Alzheimer's Disease found that overweight and obese NFL players had lower blood flow in the prefrontal cortex compared to normal-weight players.

"So I actually did a study—I published a study in the Journal of Alzheimer's Disease in my NFL group, and we looked at NFL players who were overweight or obese versus those who were at a healthy weight. The overweight and obese one had low blood flow in their prefrontal cortex" (said at 0:59:30)

Daniel Amen published a study in the Journal of Alzheimer's Disease (2020) examining regional cerebral blood flow via brain SPECT scans across BMI categories (underweight, normal weight, overweight, obese, morbidly obese). The study showed that higher BMI correlated with reduced cerebral blood flow across virtually all brain regions, including the prefrontal cortex and areas implicated in Alzheimer's disease. However, the speaker conflated this study with his earlier NFL cohort work: the published Journal of Alzheimer's Disease study on obesity and cerebral blood flow was conducted in a general adult psychiatric cohort of 17,721 participants (35,442 scans), not specifically in his NFL player group. As an observational cross-sectional imaging study from a private clinic network, the body of evidence provides low certainty.

18

Supported by research

0:01:48David Perlmutter (host)supportedhigh

Dr. Daniel Amen's research team has published more than 80 scientific articles in peer-reviewed journals.

"His research team has published more than 80 scientific articles in a variety of journals" (said at 0:01:48)

Bibliometric records confirm that Dr. Daniel G. Amen and his research team have authored and co-authored over 80 scientific articles across numerous peer-reviewed journals (such as Translational Psychiatry, PLoS ONE, Frontiers in Psychiatry, Frontiers in Neurology, and The Journal of Neuropsychiatry and Clinical Neurosciences), focusing largely on single-photon emission computed tomography (SPECT) neuroimaging across conditions including traumatic brain injury, ADHD, PTSD, depression, and suicide risk.

0:12:50David Perlmutter (host)supportedlow

Epigenetic transmission of stress and learning markers can span multiple generations in rodents and humans.

"the transmission of these epigenetic markers, if you will, can span a couple of generations, at least in rodents, and there's certainly a lot of evidence that that may well be happening in humans." (said at 0:12:50)

Animal and human literature supports the claim that epigenetic alterations associated with stress and trauma can transmit across generations. In rodent models, paternal or maternal stress induces germline epigenetic modifications (such as altered sperm microRNA profiles and DNA methylation) that persist into F1 and F2/F3 generations, causing behavioral, neuroendocrine, and metabolic phenotypes in unexposed offspring. In humans, observational studies and systematic reviews across exposed populations (such as survivors of severe trauma, war, or famine) show multi-generational associations with altered DNA methylation (e.g., NR3C1 and FKBP5 loci) and neuroendocrine dysregulation, though conclusively isolating pure germline transgenerational inheritance from in utero exposures and psychosocial transmission in humans remains an active area of ongoing research.

0:17:09Daniel Amensupportedmoderate

Social isolation is a risk factor for dementia.

"isolation is actually a risk factor for dementia" (said at 0:17:09)

Large-scale systematic reviews, meta-analyses, and umbrella reviews of prospective longitudinal cohort studies confirm that social isolation (along with loneliness and low social engagement) is an established modifiable risk factor for incident dementia, associated with an approximately 1.5-fold increased risk of developing the condition.

0:17:13Daniel Amensupportedmoderate

Social isolation is a trigger for depression.

"it's clearly a trigger for depression." (said at 0:17:13)

Extensive epidemiological and longitudinal evidence supports the claim that social isolation (and the related experience of loneliness) is a significant risk factor and precipitating trigger for the onset of depressive symptoms and major depression. A meta-analysis examining social isolation in older adults (PMID: 41578462) identified a 60% increased likelihood of depressive symptoms (pooled OR = 1.60, 95% CI: 1.39–1.84). Similarly, a systematic review and meta-analysis of prospective cohort studies (PMID: 35583561) evaluating the new onset of mental health disorders found that baseline social disconnection/loneliness more than doubled the adjusted risk of subsequent incident depression (pooled adjusted OR = 2.33, 95% CI: 1.62–3.34).

0:18:49David Perlmutter (host)supportedmoderate

Dementia is strongly associated with poor clinical outcomes in COVID-19.

"We know that dementia now looks like it's a powerful, at least association towards bad outcome." (said at 0:18:49)

Multiple large systematic reviews and meta-analyses confirm that pre-existing dementia is strongly associated with poor clinical outcomes in COVID-19, including significantly increased risk of severe disease, hospitalization, and mortality (with pooled odds ratios for mortality typically ranging between 1.6 and 5.2).

0:21:56Daniel Amensupportedmoderate

A breathing pattern of 3-4 seconds inhalation and 6-8 seconds exhalation triggers a parasympathetic nervous system response.

"we had worked on this very specific breathing pattern. It's been found to trigger a parasympathetic response, which is basically three or four seconds in, so take a deep breath, and then six to eight seconds out." (said at 0:21:56)

Paced slow breathing with prolonged exhalation (typically a 1:2 inhalation-to-exhalation ratio, such as 3-4 seconds inhaling and 6-8 seconds exhaling, corresponding to roughly 6 breaths per minute) is well-documented to enhance vagally mediated heart rate variability (HRV) and activate parasympathetic nervous system activity. Experimental studies and randomized trials demonstrate that prolonging the exhalation phase relative to inhalation increases high-frequency heart rate variability (HF-HRV) and the root mean square of successive differences (RMSSD), established physiological markers of cardiac vagal tone.

0:24:15Daniel Amensupportedlow

Dr. Amen scanned and evaluated 300 NFL players in a study starting in 2009 and found high levels of brain damage.

"I did the big NFL study starting in 2009. We scanned and treated 300 NFL players: high levels of damage." (said at 0:24:15)

Dr. Amen and colleagues initiated an ongoing longitudinal study in 2009 evaluating active and retired NFL players using SPECT perfusion neuroimaging and clinical evaluations. Published findings from this cohort reported significant hypoperfusion across 36 brain regions consistent with traumatic brain injury in 161 NFL players compared to 124 healthy controls, and a pilot open-label intervention study evaluated and treated 30 players. The certainty is low due to observational and open-label study designs.

0:28:20David Perlmutter (host)supportedmoderate

Maternal nutritional status and lifestyle factors during pregnancy influence the child's metabolic health and inflammation set points.

"the nutritional status and other lifestyle factors on the part of mom while pregnant play a role in that child's metabolic health, inflammation set points, etc." (said at 0:28:20)

Extensive epidemiological and mechanistic research under the Developmental Origins of Health and Disease (DOHaD) framework confirms that maternal nutritional status (including both undernutrition and overnutrition/obesity) and lifestyle factors during pregnancy influence fetal development, epigenetic programming, and long-term offspring outcomes, including metabolic dysfunction, adiposity, insulin resistance, and immune/inflammatory regulation.

0:30:27Daniel Amensupportedhigh

Dopamine acts directly on the nucleus accumbens in the brain.

"So dopamine is really important and it presses on the nucleus accumbens, but if you dump it, it presses too hard, just like fame." (said at 0:30:27)

The mesolimbic dopaminergic pathway is a well-established neural circuit in which dopamine-producing neurons projecting from the ventral tegmental area directly innervate the nucleus accumbens. Dopamine released into the nucleus accumbens binds to local dopamine receptors (such as D1 and D2 receptors) to regulate intracellular signaling, synaptic plasticity, reward processing, and motivated behavior.

0:29:45Daniel Amensupportedmoderate

Brain damage from head trauma increases the risk of addictive behavior and loss of behavioral control.

"You damage your brain, you're more likely to have behavior that gets out of control." (said at 0:29:45)

Traumatic brain injury (TBI) is well-documented in clinical neurotrauma and psychiatric literature to elevate the risk of behavioral dyscontrol, impulsivity, disinhibition, aggression, and addictive behaviors (such as substance use disorders and problem gambling). Systematic reviews and meta-analyses estimate that substantial proportions of TBI patients develop post-injury personality changes characterized by poor impulse control, emotional lability, and behavioral disinhibition.

0:30:00Daniel Amensupportedmoderate

Frequent massive dopamine surges lead to desensitization in the nucleus accumbens, requiring higher stimulation to experience pleasure.

"dopamine is really important and it presses on the nucleus accumbens, but if you dump it, it presses too hard, just like fame. So think of Miley or Justin. They get all of this dopamine, well, it wears it out, and then they need more and more in order to feel anything at all." (said at 0:30:00)

Extensive neuroimaging and preclinical research supports the concept that repeated, supraphysiological dopamine release in the reward circuitry (including the nucleus accumbens) leads to neuroadaptive down-regulation. Human positron emission tomography (PET) studies demonstrate that chronic excessive stimulation blunts striatal dopamine release and decreases dopamine D2 receptor availability in the nucleus accumbens and dorsal striatum. These neuroadaptations reduce baseline sensitivity to everyday rewards and require increasingly potent stimuli to achieve equivalent dopamine signaling and subjective reward.

  • supports: Addiction: beyond dopamine reward circuitry. (Proceedings of the National Academy of Sciences of the United States of America 2011) · cited 958x in the literature
    "These studies have corroborated in humans the relevance of drug-induced fast DA increases in striatum [including nucleus accumbens (NAc)] in their rewarding effects but have unexpectedly shown that in addicted subjects, drug-induced DA increases (as well as their subjective reinforcing effects) are markedly blunted compared with controls... Also, whether tested during early or protracted withdrawal, addicted subjects show lower levels of D2 receptors in striatum (including NAc)..." (abstract, passage verified)
    pubmedfull study (doi)
  • supports: The Brain on Drugs: From Reward to Addiction. (Cell 2015) · cited 1311x in the literature
    "Drugs of abuse exert their initial reinforcing effects by triggering supraphysiologic surges of dopamine in the nucleus accumbens that activate the direct striatal pathway via D1 receptors and inhibit the indirect striato-cortical pathway via D2 receptors. Repeated drug administration triggers neuroplastic changes in glutamatergic inputs to the striatum and midbrain dopamine neurons, enhancing the brain's reactivity to drug cues, reducing the sensitivity to non-drug rewards, weakening self-regulation, and increasing the sensitivity to stressful stimuli and dysphoria." (abstract, passage verified)
    pubmedfull study (doi)
0:29:10Daniel Amensupportedhigh

Dr. David Smith founded the Haight Ashbury Free Clinic.

"David Smith, who's founder of the Haight Ashbury Free Clinic and really thought of as the father of addiction medicine in the United States" (said at 0:29:10)

Historical records and scholarly literature document that Dr. David E. Smith founded the Haight Ashbury Free Medical Clinic (HAFMC) in San Francisco in 1967. The clinic was established during the counterculture movement to provide free, nonjudgmental healthcare and addiction treatment to underserved populations, cementing Smith's role as a pioneer in the development of modern addiction medicine.

0:45:53David Perlmutter (host)supportedlow

Hand sanitizers contain solvents that strip the fat layer on the skin that acts as a protective barrier against toxins.

"First, that there's a lot of solvents in these hand sanitizers before we even get to the toxins, so we're stripping away the fat on our skin, the fat layer that is there to protect us against the ability of these toxins to get in. So breaking down the barrier protection first of all" (said at 0:45:53)

Alcohols used as active solvent ingredients in hand sanitizers (such as ethanol and isopropanol) are well-characterized chemical penetration enhancers. The stratum corneum lipid matrix—composed of ceramides, cholesterol, and free fatty acids—forms the primary physical barrier preventing external molecules from penetrating the skin. Mechanistic and biophysical studies demonstrate that exposure to alcohols can extract intercellular lipids (such as free fatty acids), alter lipid bilayer fluidity, and reduce the permeation energy barrier, thereby increasing skin permeability to external substances. However, standard commercial hand sanitizers are commonly formulated with humectants (such as glycerin) to mitigate barrier disruption, and typical use involves transient exposure rather than continuous solvent soaking.

0:48:39David Perlmutter (host)supportedvery low

Research by Molly Fox suggests that higher parasitic burden correlates with lower risk for diseases such as Alzheimer's disease.

"That there is actually an upside to parasites in human immune responsiveness that's balancing and it's actually good for us. We could talk about that in terms of risk in cultures that have higher parasitic burden related to things even as seemingly disparate as Alzheimer's disease—some work by a woman named Molly Fox, I had a chance to interview her." (said at 0:48:39)

The speaker accurately describes the published research by Molly Fox and colleagues (Fox et al., 2013). In an ecological study assessing 192 countries, Fox and co-authors evaluated the hygiene hypothesis in relation to Alzheimer's disease (AD) and found that lower pathogen prevalence and higher sanitation levels were significantly associated with higher age-standardized AD disability-adjusted life-year (DALY) rates. However, because this research is based on country-level ecological correlations rather than individual-level longitudinal or clinical trial data, the overall certainty of evidence for a causal or protective effect remains very low.

1:00:15Daniel Amensupportedhigh

In the United States population, 72% are overweight, 42% are obese, and 50% are diabetic or pre-diabetic according to published JAMA data.

"And what is it, 72% of the population is now overweight, 42% obese, 50% are diabetic or pre-diabetic, that was published in JAMA." (said at 1:00:15)

Published nationally representative epidemiological data (primarily from the National Health and Nutrition Examination Survey [NHANES], frequently reported in JAMA and CDC publications) support these figures for US adults. In a landmark JAMA analysis of NHANES data (Menke et al., 2015), the unadjusted prevalence among US adults was 14.3% for total diabetes (diagnosed and undiagnosed) and 38.0% for prediabetes, totaling 52.3% (over 50%) with diabetes or prediabetes. Similarly, NHANES surveillance data show that approximately 71% to 73% of US adults are classified as overweight or obese (BMI ≥25 kg/m²), with adult obesity prevalence (BMI ≥30 kg/m²) reaching approximately 42.4%.

1:00:39Daniel Amensupportedhigh

Adipose tissue converts testosterone into forms of estrogen.

"If you're overweight, your hormone balance isn't right because fat takes healthy testosterone, turns it into unhealthy forms of estrogen." (said at 1:00:39)

Adipose tissue expresses the enzyme aromatase (CYP19A1), which catalyzes the peripheral conversion of androgens, such as testosterone and androstenedione, into estrogens (primarily 17β-estradiol and estrone). In overweight and obese individuals, increased adipose tissue volume and elevated aromatase activity lead to higher conversion rates of testosterone into estrogens, contributing to obesity-related male hypogonadism and altered sex hormone balance.

1:00:52Daniel Amensupportedlow

A published study by Daniel Amen examining 35,000 brain scans found a linear correlation where higher body weight was associated with decreased blood flow across virtually every brain region.

"I published a study last year on 35,000 scans showing there was, David, a linear correlation: as your weight went up, blood flow to virtually every area of your brain went down." (said at 1:00:52)

A 2020 cross-sectional study by Daniel Amen and colleagues analyzed 35,442 brain SPECT scans from 17,721 adults in a psychiatric clinic cohort across different BMI categories. The study reported that higher BMI was significantly correlated with decreased cerebral blood flow (hypoperfusion) during both resting and concentration tasks across virtually all 128 brain regions examined. Because this was a retrospective, cross-sectional observational study in a self-selected clinical population, it demonstrates association rather than direct causation.

0:55:35Daniel Amensupportedhigh

Chronically high blood sugar damages blood vessels, causing them to lose flexibility and become brittle and prone to breaking.

"Which is an emergency, because when your blood sugar is chronically high, it erodes your blood vessels, making them more likely to lose their flexibility, be brittle, and more likely to break." (said at 0:55:35)

Published literature confirms that chronic hyperglycemia drives vascular damage through multiple well-characterized pathways, including endothelial dysfunction, oxidative stress, advanced glycation end-product (AGE) cross-linking of extracellular matrix proteins, and vascular smooth muscle calcification. These pathophysiological alterations directly result in loss of arterial compliance (increased arterial stiffness) and structural vessel wall fragility, which predisposes to microvascular and macrovascular injury.

2

Corroborated by web sources

0:01:35David Perlmutter (host)corroborated (web)

Amen Clinics has a database of more than 170,000 brain scans from patients across 150 countries related to behavior.

"Amen Clinics has the world's largest database of brain scans that are related to things like behavior, totaling more than 170,000 scans on patients from 150 countries." (said at 0:01:35)

No published record matching the claim that Amen Clinics holds a database of more than 170,000 brain scans from patients across 150 countries was located; this does not prove the claim false. While peer-reviewed studies published by researchers associated with the clinics utilize large multisite retrospective clinical databases of single-photon emission computed tomography (SPECT) scans from psychiatric outpatients, the specific registry figures and international breakdown cited in promotional statements have not been independently verified in peer-reviewed scientific publications.

corroborated by web sources

Amen Clinics and promotional materials for founder Dr. Daniel Amen report holding the world's largest functional brain scan database related to behavior. Official clinic publications and biographical profiles cite having accumulated more than 170,000 brain SPECT scans from patients across 150 countries.

amenclinics.comdrperlmutter.comfacebook.com

0:01:54David Perlmutter (host)corroborated (web)

Discover Magazine named Dr. Daniel Amen's research as one of the Top Stories in Science in 2015.

"Discover Magazine has named his research as one of the Top Stories in Science back in 2015." (said at 0:01:54)

No published record matching the claim that Discover Magazine named Dr. Daniel Amen's research as one of the Top Stories in Science in 2015 was located; this does not prove the claim false.

corroborated by web sources

Discover Magazine listed Dr. Daniel Amen's 2015 research using SPECT brain imaging to distinguish between post-traumatic stress disorder and traumatic brain injury as #19 on its list of the top 100 stories in science for 2015.

amenclinics.comdiscovermagazine.comcision.com

2

No source found (not proven false)

0:50:20Daniel Amenunverifiedvery low

Tom Brady gets nine hours of sleep per night and avoids gluten, dairy, corn, soy, artificial dyes, sweeteners, and sugar.

"Gets nine hours of sleep, he doesn't eat gluten, dairy, corn, soy, artificial dyes, sweeteners, sugar. I mean, his diet is really clean" (said at 0:50:20)

No published record matching the specific daily sleep duration and dietary exclusions of Tom Brady was located in the scientific literature; this does not prove the claim false. While the described nutritional rules align broadly with public descriptions of the commercial "TB12 Method" lifestyle program, individual lifestyle regimens of specific athletes are not documented or evaluated in peer-reviewed biomedical research.

1:00:00Daniel Amenunverifiedvery low

Former NFL player Ed White lost 150 pounds participating in Dr. Daniel Amen's NFL weight loss group.

"And we did an NFL weight loss group, which was so much fun for me. I had Big Ed White become Little Ed White. He lost 150 pounds, which was really fun." (said at 1:00:00)

No published record matching the claim that former NFL player Ed White lost 150 pounds in Dr. Daniel Amen's weight loss group was located; this does not prove the claim false. Dr. Amen published a study detailing an open-label multi-component intervention in 30 retired NFL players that included weight loss where appropriate (PMID 21615001), but individual player identities and specific weight loss figures such as 150 pounds were not reported in peer-reviewed publications.

Unverified means no publication matching the claim was located; it does not prove the claim false. Spotted an error? See the corrections policy - disputes from the people quoted are prioritized.