Sánchez-López · Archives of medical research 2018 · double-blind randomized placebo-controlled trial · n=36

Efficacy of Melatonin on Serum Pro-inflammatory Cytokines and Oxidative Stress Markers in Relapsing Remitting Multiple Sclerosis.

Cited 100 times in the scientific literature.

Level 2 - randomized trial

Individual randomized, double-blind, placebo-controlled trial

PubMed 30595364 · doi:10.1016/j.arcmed.2018.12.004 · record verified 2026-08-30

What was done

A double-blind, randomized, placebo-controlled trial evaluated oral melatonin (25 mg daily for 6 months) as an adjunct therapy in 36 patients diagnosed with relapsing-remitting multiple sclerosis (RRMS) who were concurrently treated with Interferon β-1b (IFNβ-1b). Serum inflammatory cytokines, oxidative stress markers, clinical efficacy outcomes, and adverse effects were assessed.

What was found

Compared to placebo, melatonin administration led to statistically significant reductions in serum concentrations of inflammatory cytokines and oxidative stress markers: TNF-α decreased by 18% (p < 0.05), IL-1β by 34.8% (p < 0.05), IL-6 by 34.7% (p < 0.05), lipoperoxides (LPO) by 39.9% (p < 0.05), and nitric oxide catabolites (NOC) by 24% (p < 0.05). There was no significant difference between groups in clinical efficacy outcomes or adverse effect rates.

Why it matters

Melatonin may serve as an effective adjuvant antioxidant and anti-inflammatory agent for biochemical modulation in RRMS, though its ability to improve functional or clinical neurological outcomes remains unproven.

Limits

The study had a very small sample size (n = 36) and a relatively short follow-up duration (6 months). All participants were on background IFNβ-1b therapy, limiting generalizability to untreated patients or other disease-modifying therapies. The abstract reports biochemical changes but notes no detectable clinical benefits, and specific clinical endpoints measured are not detailed.

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